Molecular Biology

• Cloning and expression of gene in E. coli, yeasts and mammalian cells   • Design and construction of synthetic genes   • Development of novel expression vectors for yeasts   • PCR/RT-PCR analysis

Fermentation

• Development of microbial fermentation process up to 1000 L scale   • Optimization of fermentation under different modes of operation (pH stat, DO stat, Chemostat, intermittent and continuous fed batch)d   • Development of novel feeding strategies for enhancing expression of heterologous proteins   • High cell density fermentation of Pichia pastoris and E. coli

ScFv development

• Phage display technology and affinity maturation for the development of high affinity Fabs and ScFv.   • Engineering of ScFv fragments (VH-VL & VL - VH) and optimization for high level expression in Pichia and E. coli.   • Fermentation process development in diverse host systems including yeast and bacteria.   • Purification and downstream process development.

Scale up and technology transfer

• cGMP MCB / WCB Banking   • Pilot scale production   • SOPs and documentation